Both managers have selection dilemmas ahead of the match, with Pep Guardiola's Barca side particularly hard hit by injuries.
Skipper Carles Puyol is out, Andres Iniesta is in doubt, while French left-back Eric Abidal is still recovering from surgery to remove a tumour from his liver.
Defenders Maxwell and Adriano are both injured, and to further stretch Guardiola's reserves at the back, Gabriel Milito and Martin Montoya will both miss out after they were hurt in the match with Real Sociedad.
Milito suffered a calf strain while Montoya - in his full match for the club - suffered a broken collarbone.
Mourinho has defensive headaches of his own, with Sergio Ramos and Pepe both suspended, but central defender Ricardo Carvalho should be back after his one-match ban.
Midfielder Sami Khedira is out through injury, but Mourinho could inject Kaka into the fray with the need for goals paramount, after the influential Brazilian was conspicuous by his absence in the first leg.
